Chicken natural killer cells - Target cell susceptibility and modulation by interferon inducers*


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Authors

  • R Govindarajan
  • P C Verma
  • T N Jaiswal

Keywords:

Chicken, Interferon inducers, Modulation, Natural killer cells, Target cells

Abstract

Cytotoxicity assays were carried out using splenic and peripheral blood non-adherent mononuclear cells as effectors on xenogeneic (vero and BHK-21 cells) and allogeneic (CEF) targets by MIT colorimetric assay and chromium release assay (CRA). CRA was unreliable due to high spontaneous release, MIT cytotoxicity assays clearly showed the susceptibility of xenogeneic targets to chicken natural cell-mediated cytotoxicity, Healthy, secondary CEF cultures were also susceptible to lysis, Non-adherent, T and B depleted effectors were more efticient than non-adherent non-depleted cells at any given E:T ratio suggesting NK cell-mediated lysis, Peripheral blood exhibited more NK activity than their splenic counter parts, Lymphokine activation of killer cells resulted in increased cytotoxic activity at higher E:T ratios, Peripheral blood NK cell activity on vero cells was significantly Increased after 48 hr of vaccination with R2B strain of Ranikhet disease vaccine than that of at 24 hr postvaccination, A role for interferons is suggested, Synthetic RNA poly (I:C) had no augmentative effect on splenic NK cell activity after 3 hr of intra-peritoneal injection.

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How to Cite

Govindarajan, R., Verma, P. C., & Jaiswal, T. N. (2012). Chicken natural killer cells - Target cell susceptibility and modulation by interferon inducers*. The Indian Journal of Animal Sciences, 68(11). https://epubs.icar.org.in/index.php/IJAnS/article/view/22411