A purified tachyzoite protein based ELISA for the sero-surveillance of Toxoplasma gondii specific antibodies in domestic ruminants


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Authors

  • HARKIRAT SINGH
  • A K TEWARI
  • A K MISHRA
  • B R MAHARANA
  • J R RAO

Keywords:

Cattle, ELISA, Goat, Sheep, Sero-Surveillance, Toxoplasma gondii

Abstract

Toxoplasmosis, an immensely significant zoonotic disease, is caused by coccidian Toxoplasma gondii. Data on
prevalence of the parasite in economically important livestock species from India is scanty. An antibody detection
ELISA specific for T. gondii was laboratory standardized using purified tachyzoite lysate antigen of T. gondii (RH
strain). Sera samples (168) from sheep (60), goat (63) and cattle (45) were procured from regions of Uttar Pradesh and
West Bengal for assessing the sero-surveillance of T. gondii specific antibodies. Among the small ruminants, 31.66%
and 17.46% of the total sera samples collected from sheep and goats, respectively, were detected positive for T. gondii
specific antibodies. The sero-surveillance of toxoplasmosis among cattle was 28.88%. The study has relevance for
developing control strategies against this zoonotic organism.

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Author Biographies

  • HARKIRAT SINGH
    Assistant Professor, Department of Veterinary Parasitology, College of Veterinary Science, GADVASU, Ludhiana 141 004.
  • A K TEWARI
    Senior Scientist, Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh 243 122 India
  • A K MISHRA
    Principal Scientist (Retd.) Division of Parasitology.Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh 243 122 India

  • B R MAHARANA
    Assistant Professor, Department of Parasitology, College of Veterinary Science and Animal Husbandry, Junagadh Agricultural University, Junagadh, Gujarat.Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh 243 122 India

  • J R RAO
    Consultant, PGD-TMA, NAARM, Hyderabad Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh 243 122 India

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Issue

Vol. 83 No. 1 (2013)

Section

Articles

License

The copyright of the articles published in The Indian Journal of Animal Sciences is vested with the Indian Council of Agricultural Research, which reserves the right to enter into any agreement with any organization in India or abroad, for reprography, photocopying, storage and dissemination of information. The Council has no objection to using the material, provided the information is not being utilized for commercial purposes and wherever the information is being used, proper credit is given to ICAR.

How to Cite

SINGH, H., TEWARI, A. K., MISHRA, A. K., MAHARANA, B. R., & RAO, J. R. (2013). A purified tachyzoite protein based ELISA for the sero-surveillance of Toxoplasma gondii specific antibodies in domestic ruminants. The Indian Journal of Animal Sciences, 83(1). https://epubs.icar.org.in/index.php/IJAnS/article/view/26425