Nucleotide sequence analysis of the viral envelope protein gene (P32) region of sheep poxvirus strains from India
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Authors
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S Rajamuthu
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M Parthiban
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T M A Senthil Kumar
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Wilson Aruni
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S Senthil Kumar
Keywords:
Basic local alignment search tool, Ranipet strain, Roumaninan–Fanar strain, Polymerase chain reaction, Sheep poxvirusAbstract
Local field strains (7) of sheep poxvirus from different districts of Tamil Nadu, India, and 2 vaccine strains were characterized by sequencing studies to find out the marker gene in an attempt to differentiate the field and vaccine strains. The full length viral envelope protein gene (P32) of sheep poxvirus was amplified and sequenced. The sequence studies revealed that the local field strains possessed 100% and 99.31% homology with Ranipet and Roumanian–Fanar vaccine strains respectively. Sheep poxvirus cannot be differentiated based on the envelope protein nucleotide sequence level in any field outbreaks.
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