Genetic variability and bottleneck analyses of Kanniadu goat breed based on microsatellite markers

Abstract

Genetic variation at 25 microsatellite loci was examined in Kanniadu goats of Tamil Nadu. The Kanniadu goat is a prolific meat breed thriving well in the tropical draught conditions. The observed number of alleles ranged from 5 (RM4) to 13 (RM088, OarE129) with an average value of 8.64±0.48. The effective number of alleles ranged from 1.45 (ILSTS34) to 7.89 (ILSTS033 and OMHC1) with the overall mean value of 4.22±0.34. The average observed and expected heterozygosity values were 0.53±0.03 and 0.73±0.02, respectively. The polymorphic information content value ranged from 0.30 to 0.86. These high values of PIC indicated higher polymorphism in the breed. Within population inbreeding estimate (Fis = 0.25) showed moderate level of inbreeding, which warranted adoption of appropriate breeding strategies under field conditions. The high level of genetic variability, however, suggested the scope for further genetic improvement of Kanniadu goats.