Immune response of rabbits immunized with outer membrane proteins (porins) of Salmonella dublin 51

Abstract

Porins complexed with polysaccharide of Salmonella dublin 51 used for immunization of rabbits proved non· toxic. Agglutinin titre ranged from 1:40 to 1:320 against '0' 9, 12 antigen. Titre against porins by dot-ELISA was 1:100-1 :400. Antiporins in the serum samples of 2 rabbits were demonstrable at 5 weeks
postimmunizution bycounter-immunoelectrophoresis (Cm), while it was detected in the 4 rabbits at 6 weeks post-challenge before sacrifice by both immunodiffusion ami ClE. The immediatetype skin hypersensitivity reaction was 12.00. 15.00mm (skin thickness) at 6 hr (Peak skin reaction) post-antigen inoculution in the rabbits, at 5 weeks Post immunization. Skin biopsy collected at 6 hr (peak skin reaction) post-antigen inoculation revealed infiltration of neutrophils. The antiporin in the serum samples of all the rabbits were also deteoted by dot ELISA at 6 weeks post-challenge before sacrifice. The cell-mediated immune response (Icucocytemignllion inhibition and delayed type of skin hypersensitivity reaction) in the immunized rabbits was insignificant. The percentage protection of the immunized rabbits was 66.66% (statistically significant) against oral challenge.