Detection of rtxA gene and other virulence genes in non-O1 and non-O139 Vibrio cholerae isolated from ornamental fishes in India


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Authors

  • THANGARAJ RAJA SWAMINATHAN
  • GAURAV RATHORE
  • NEERAJ SOOD
  • WAZIR SINGH LAKRA

Keywords:

Goldfish, Koicarp, Ornamental fish, PCR, Vibrio cholerae

Abstract

Vibrio cholerae non-O1 and non-O139 serogroups (16) were isolated from koi carp, goldfish and aquarium water during 2004–2005. All the isolates were confirmed by amplifying the 300bp fragment in the 16S–23S rRNA intergenic spacer region. By several PCR assays, it was determined that none of the non-O1 and non-O139 isolates were positive for ctxA, zot, ace and tcpA genes. But all the isolates were positive for the toxR, ompU and rtxA genes.

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Issue

Vol. 78 No. 7 (2008)

Section

Articles

License

The copyright of the articles published in The Indian Journal of Animal Sciences is vested with the Indian Council of Agricultural Research, which reserves the right to enter into any agreement with any organization in India or abroad, for reprography, photocopying, storage and dissemination of information. The Council has no objection to using the material, provided the information is not being utilized for commercial purposes and wherever the information is being used, proper credit is given to ICAR.

How to Cite

SWAMINATHAN, T. R., RATHORE, G., SOOD, N., & LAKRA, W. S. (2011). Detection of rtxA gene and other virulence genes in non-O1 and non-O139 Vibrio cholerae isolated from ornamental fishes in India. The Indian Journal of Animal Sciences, 78(7). https://epubs.icar.org.in/index.php/IJAnS/article/view/3312