Haematologieal and biochemical responses in native sheep experimentally infected dth bluetongue Grus serotype-23

Abstract

Haematological and sero-biochemical responses of sero-negative native sheep to bluetongue virus serotype-23 were studied and compared with the control group. Two test groups, each containing 8 animals were inoculated with 6xIOSF TCID,, of BTV-23 serotype separately by intravenous (GrIV) and intradermal (GrID) routes. The blood and serum samples were analyzed at 0, 3, 7, 11, 15 and 20 days post-infection (DPI). Significant changes were observed in all the hematological and biochemical parameters studied. Marked leucopenia was observed on 3 DPI in GrID while leucocytosis in GrIV group. Significant leucocytosis was observed during 11 to 15 DPI in both the infected groups. Significant thrombocytopenia was observed during 3 to 15 DPI whereas significantly low PCV and Hb values were observed between 3 and 11 DPI in both test groups. Differential leucocytes count revealed significantly low lymphocyte percentage on day 3 and high on day 15 in both the infected groups. Under enzyme biochemical studies, ALT registered significantly high values during 7 to 20 DPI in both the test groups, AST during 11 DPI in GrIV and 7 to 11 DPI in GrID, ALP during 3 to 11 DPI in GrIV and 7 DPI in GrID, and CK during 7 to 15 DBI in both the test groups. These findings indicated the damage to various soft tissue organs and muscles as a sequel to vascular endothelial damage and subsequent inflammatory changes with associated ischemia and necrosis