Anti-tick effects sf 34kDa glycoprotein of Hyalomma anatolicum anatolicum in combination with adjuvant

Abstract

immunization of Bas taurus x B. indicus calves by 34 k D3 glycoprotein combined with surfactant adjuvant, saponin, against Hyalloma anatolicum anatolicum conferred significant protection against homologous challenge infestations of larvae and adults. The per cent reduction of challenged larvae (DT),% reduction in moulting of engorged larvae (MO) and efficacy per cent of the antigen agbinst challenged larvae (E) were calculated as 32, 35 and 56, respectively. In adults, the percent reduction of challenged adults (DT), the per cent reduction in mean weight of eggs of tick fed on immunized and control animals (DO), the per cent reduction of mean weight of adult females dropped from immunized and control animals (DR) and efficacy per cent of the antigen against challenged adults (E) were calculated as 42, 12.15, 17.6 and 52.44 per cent, respectively. A significantly higher level of E% obtained after repeated challenge infestations proved the additive effect of 34 kDa antigen and antigens of salivary glands. A direct correlation between antibody response against 34kDa glycoprotein and protection against infestations was observed. Significantly high level of variation in the entomological data of ticks fed on immunized and control animals was recorded. The effect of h e 34 kDa antigen in combination with saponin in Limiting the H.a. anatolicum infestation boosted the level of protection.