Antigenic characterization of an Indian isolate of infectious bursal disease virus (IBDV-P/AD/81) with overseas monoclonal antibodies


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Authors

  • S C DAS
  • G C RAM
  • K C VERMA
  • J M KATARIA

Abstract

The Indian isolate of infectious bursal disease virus, viz. IBDV-P/AD/81. when reacted with monoclonal antibodies (MoAb) P40/IV/B4 and P40/B1 (directed against 40 kd (VP2) polypeptide of chicken isolate Cu-I) showed maximum reactivity in ELISA and neutralization, and formed precipitin bands in AGPT indicating its antigenic similarity with exotic chicken isolate Cu-l. MoAb P32/E1 (directed against 32 kd (VP3) polypeptide of chicken isolate Cu-l), though highly reacted in ELISA but did not show any neutralization and was not precipitating. The virus did not react in ELISA. AGPT and neutralization with MoAb m/AK/T/41/A2 (directed against 41 kd (VP2) polypeptide of turkey isolate 23/82) indicating its antigenic dissimilarity within the exotic turkey isolate 23/82.

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Author Biographies

  • S C DAS
    Scientist Division of Animal Health CSWRI, Avikanagar (Via:Jaipur), Rajasthan 304 501.
  • G C RAM
    Scientist Incharge, Immunology Section, Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh 243 122

  • K C VERMA
    Principal Scientist, Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh 243 122
  • J M KATARIA

    Scientist (S.S.), Division of Avian Diseases. Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh 243 122

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Issue

Vol. 65 No. 9 (1995)

Section

Articles

License

The copyright of the articles published in The Indian Journal of Animal Sciences is vested with the Indian Council of Agricultural Research, which reserves the right to enter into any agreement with any organization in India or abroad, for reprography, photocopying, storage and dissemination of information. The Council has no objection to using the material, provided the information is not being utilized for commercial purposes and wherever the information is being used, proper credit is given to ICAR.

How to Cite

DAS, S. C., RAM, G. C., VERMA, K. C., & KATARIA, J. M. (2013). Antigenic characterization of an Indian isolate of infectious bursal disease virus (IBDV-P/AD/81) with overseas monoclonal antibodies. The Indian Journal of Animal Sciences, 65(9). https://epubs.icar.org.in/index.php/IJAnS/article/view/33624