SDS-polyacrylamide-gel electrophoresis of sperm proteins of buffalo and cattle bulls


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Authors

  • B A KULKARNI
  • R K RUPAL
  • UMASHASHI C HEGOE

Keywords:

Buffalo bulls, Cattle bulls, SDS-PAGE, Sperm proteins

Abstract

Sperm protein patterns in buffalo and cattle breeding bulls were analysed by SDS-PAGE. Buffalo and cattle bull spermatozoa had 21 and 23 protein bands of varying intensities and molecular weight ranging from> 100 to 13 KDa. Distinct species differences in sperm protein patterns were observed. A major protein of molecular weight 16 KDa and a broad protein band having molecular weight below 14 KDa were present in cattle spermatozoa but absent in buffalo spermatozoa. Individual variations and distinct breed differences in sperm protein patterns of Hiolstein-Friesian. Jersey and crossbred cattle bulls and Murrah and Surti buffalo bulls were observed.

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Author Biographies

  • B A KULKARNI
    Emeritus Scientist ( ICAR), Konkan Krishi Vidyapeeth, Bombay Campus, Mumbai, Maharashtra 400 012
  • R K RUPAL
    Department of Physiology and Biochemistry,
    Bombay Veterinary College, Parel Mumbai 400 012
  • UMASHASHI C HEGOE
    Deputy Director, Institute for Research in Reproductio (ICMR) Parel, Mumbai, Maharashtra 400 012.

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Issue

Vol. 67 No. 2 (1997)

Section

Articles

License

The copyright of the articles published in The Indian Journal of Animal Sciences is vested with the Indian Council of Agricultural Research, which reserves the right to enter into any agreement with any organization in India or abroad, for reprography, photocopying, storage and dissemination of information. The Council has no objection to using the material, provided the information is not being utilized for commercial purposes and wherever the information is being used, proper credit is given to ICAR.

How to Cite

KULKARNI, B. A., RUPAL, R. K., & HEGOE, U. C. (2013). SDS-polyacrylamide-gel electrophoresis of sperm proteins of buffalo and cattle bulls. The Indian Journal of Animal Sciences, 67(2). https://epubs.icar.org.in/index.php/IJAnS/article/view/34193