Immunization of cattle against tick, Boophilus microplus


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Authors

  • S GHOSH
  • M H KHAN

Keywords:

Boophilus microplus, Crossbred calves, , Enzyme linked immunosorbent assay, Larval antigen, Tick extract supernatant antigen

Abstract

Two antigens, viz. tick extract supernatant antigen (TESA) and larval antigen (LA), were prepared from 109 each of partially fed adults and unfed larvae of Boophilus microplus. Calves in 2 batches of 6 and 8 were immunized with 53 and 45 mg of protein in 3 divided doses. With enzyme linked immuilosorbent assay (ELISA), a significant antibody (P<0.01), and significant (P <0.01) changes in the weight of engorged females and egg masses were noticed with both the antigens. The purification of TESA yielded 3 peaks. Whole sera showed precipitation against peak 1 o,nly. In another ELISA test with peak l(Pl) fraction ofTESA, significant (p<0.05) teaction with anti-LA sera indicated that the protective antigen (s)was common in larvae and adults of B.microplus.

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Author Biographies

  • S GHOSH
    Scientist, Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh 243 122

  • M H KHAN
    Principal Scientist, Division of Parasitology, Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh 243 122

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Issue

Vol. 67 No. 3 (1997)

Section

Articles

License

The copyright of the articles published in The Indian Journal of Animal Sciences is vested with the Indian Council of Agricultural Research, which reserves the right to enter into any agreement with any organization in India or abroad, for reprography, photocopying, storage and dissemination of information. The Council has no objection to using the material, provided the information is not being utilized for commercial purposes and wherever the information is being used, proper credit is given to ICAR.

How to Cite

GHOSH, S., & KHAN, M. H. (2013). Immunization of cattle against tick, Boophilus microplus. The Indian Journal of Animal Sciences, 67(3). https://epubs.icar.org.in/index.php/IJAnS/article/view/34250