Typing of leptospiral serovars by DNA restriction enzyme analysis


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Authors

  • A SENTHIL KUMAR
  • P RAMADASS
  • K NACHIMUTHU

Keywords:

Rats, Leptospirosis, Leptospiral serovars, DNA restriction enzyme

Abstract

DNA restriction endonucleases have been used for characterizing the various leptospiral serovars. Reference strains (13) and rat isolate RI were selected for this study. DNA samples were prepared and subjected to restriction enzyme analysis with different enzymes. Complete digestion of the samples was obtained with Bam HI, Eco RI, Hae III, Hha I and Hind III: The enzymes Hae III and Hha I produced good resolution, among the patterns produced by the enzyme Hae III facilitated clear differentiation between different serovars. The rat isolate RI restriction enzyme map was similar in pattern with the reference strain Veldrat Batavia 46 of the serovarjavanica.

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Author Biographies

  • A SENTHIL KUMAR
    Research Scholar, Tamil Nadu Veterinary and Animal SCiences University, Chennai, Tamil Nadu 600 007
  • P RAMADASS
    Professor, Tamil Nadu Veterinary and Animal SCiences University, Chennai, Tamil Nadu 600 007
  • K NACHIMUTHU
    Professor and Head, Department of Animal Biotechnology, Madras Veterinary College.

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Issue

Vol. 67 No. 6 (1997)

Section

Articles

License

The copyright of the articles published in The Indian Journal of Animal Sciences is vested with the Indian Council of Agricultural Research, which reserves the right to enter into any agreement with any organization in India or abroad, for reprography, photocopying, storage and dissemination of information. The Council has no objection to using the material, provided the information is not being utilized for commercial purposes and wherever the information is being used, proper credit is given to ICAR.

How to Cite

KUMAR, A. S., RAMADASS, P., & NACHIMUTHU, K. (2013). Typing of leptospiral serovars by DNA restriction enzyme analysis. The Indian Journal of Animal Sciences, 67(6). https://epubs.icar.org.in/index.php/IJAnS/article/view/34578