Thermostability of rinderpest vaccine virus after adaptation to Vero cells

Abstract

Stability of Vera-cell-adapted rinderpest vaccine virus with 2 different stabilizers, viz. 5% lactalbumin hydrolysate (LAR) plus 10%, sucrose (LS stabilizer) and 5% LAH plus 10% lactose (LL stabilizer), was tested lit various temperatures. At 4^oC, no loss in infective titres was found after 3 months of storage. At 25^oC on storage for 29 days, loss of infectivity was nonsignificant. A significant (P < 0.05) linear decline in the titres was observed when stored at 37° C for 21 days as well as at 45° C for 8 days. Based on declining degradation curves observed at different temperatures, the vaccine is expected to reach the minimum W.H.O. requirement of 2.5 log₁₀ TICD₅₀ after storage for approximately 305 days (LS) and 123 gays (LL) at 25°C; 28 days (LS) and 48 days (LL) at 37° C; and 3 days (LS) and 7 days (LL) at 45° C. Stabilizer LL was10und to be marginally superior to LS at 3 r and 45° C.