Antigenic characterization of foot-and-mouth disease virus type O strains isolated from outbreaks in vaccinated herds


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Authors

  • S A WANI
  • K L AHUJA
  • S PRASAD
  • ARVIND KUMAR

Keywords:

Antigenic characterization, FMD virus, Foot-and-mouth disease

Abstract

The extent of antigenic relationship of foot-and-mouth-disease (FMD) virus field strains with reference to (vaccine) strain was assessed. Three type 0 field strains, isolated from outbreaks in vaccinated herds of different areas and periods, were selected. The isolates were characterized by sandwich-enzyme-linked immunosorbent assay (ELISA) and in vivo cross-protection tests in guineapigs. In one-way testing by sandwich ELISA, field isolates were reacted with anti-146S reference serum and the relationship was revealed by calculation ofr-values. In reverse direction testing, the reference strain was reacted with anti-146S sera against field isolates followed by calculation of r-values. The r-values obtained in both the directions were >0.25. This suggested a relationship of the reference strain with field isolates and vice versa. The reference strain gave 100% protection to guineapigs against challenge with all the 3 field isolates. This showed a good correlation between in vitro sandwich ELISA and in vivo cross-protection tests.

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Author Biographies

  • S A WANI
    Associate Professor- cum-Senior Scientist, Department of Microbiology & Immunology, Faculty of Veterinary
    Sciences and Animal Husbandry, Shuhama P. B. No. 135, O. P. O. Srinagar 190 001.
  • K L AHUJA
    Senior Epidemiologist, CCS Haryana Agricultural University, HisQT, Haryana J 25 004

  • S PRASAD
    Ex- Senior Virologist, CCS Haryana Agricultural University, HisQT, Haryana J 25 004

  • ARVIND KUMAR
    Virologist, Department of Veterinary Microbiology, CCS Haryana Agricultural University, HisQT, Haryana J 25 004


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How to Cite

WANI, S. A., AHUJA, K. L., PRASAD, S., & KUMAR, A. (2013). Antigenic characterization of foot-and-mouth disease virus type O strains isolated from outbreaks in vaccinated herds. The Indian Journal of Animal Sciences, 67(7). https://epubs.icar.org.in/index.php/IJAnS/article/view/34694