Quantitation of immunoglobulins (IgG, IgM and IgA) in serum and lacteal secretion of buffaloes (Bubalus bubalis)

Abstract

Buffalo immunoglobulins (Igs) IgG₁, IgG₂ igM and IgA were prepared and monospecific antiserum was raised against each. These were utilized to quantitate Igs in 66 Sera samples and lacteal secretions of 3 buffaloes from 0 to 84 hr postpartum. The concentration of Igs in serum was unaffected by age of the animals and their distribution was normal. The mean IgG1₁, IgG₂, IgG, IgM and IgA concentration in serum was 17.46, 14.08,31.54,2.92 and 0.27 mg/ml, respectively. The corresponding values in lacteal secretions of the 3 buffaloes immediately postpartum were 31.62, 1.96,33.58,0.52 and 0.27 mg/ml, respectively. Thereafter the Ig ,concentrations declined sharply, 7-fold by 24 hr and more than 30-fold by 84 hr postpartum.