Use of purified soluble antigens of sheep pox-virus in serodiagnosis*


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Authors

  • T V S RAO
  • B S NEGI
  • M P BANSAL

Keywords:

Agar-gel precipitation, Affinity chromatography, Antigens, Counter immunoelectrophoresis, Indirect and Immunocapture ELISA, Reverse phase passive haemagglutination, Sheep poxvirus, Sheep-pox diagnosis, Soluble antigens

Abstract

A soluble antigen fraction of sheep poxvirus was isolated by ultracentrifugation of the clarified scab suspension prepared from the pox lesions of experimentally infected sheep. The residual infectivity studies showed these soluble antigens to be noninfectious. They were subsequently purified by subtractive immuno-affinity chromatography to remove healthy skin proteins. The purified soluble antigen fraction and its antiserum were then evaluated for the diagnosis of sheep-pox by using various serological tests including agar-gel precipitation, counter immunoelectrophoresis, reverse phase passive haemagglutination and enzyme-linked immunosorbent assays. The utility of soluble antigens is discussed.

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Author Biographies

  • T V S RAO
    Scientist, Indian Veterinary Research Institute Campus, Mukteswar-Kumaon, Uttar Pradesh 263 138

  • B S NEGI
    Head and Principal Scientist, Division
    of Virology, Indian Veterinary Research Institute Campus, Mukteswar-Kumaon. Uttar Pradesh 263 138

  • M P BANSAL
    Incharge and Principal Scientist, Immunology Section, IVRI, Izatnagar243 122, Indian Veterinary Research Institute Campus, Mukteswar-Kumaon. Uttar Pradesh 263 138

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Issue

Vol. 67 No. 8 (1997)

Section

Articles

License

The copyright of the articles published in The Indian Journal of Animal Sciences is vested with the Indian Council of Agricultural Research, which reserves the right to enter into any agreement with any organization in India or abroad, for reprography, photocopying, storage and dissemination of information. The Council has no objection to using the material, provided the information is not being utilized for commercial purposes and wherever the information is being used, proper credit is given to ICAR.

How to Cite

RAO, T. V. S., NEGI, B. S., & BANSAL, M. P. (2013). Use of purified soluble antigens of sheep pox-virus in serodiagnosis*. The Indian Journal of Animal Sciences, 67(8). https://epubs.icar.org.in/index.php/IJAnS/article/view/34868