Concentration and purification of infectious bursal disease virus (IBDV) by sucrose density gradient centrifugation


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Authors

  • S C DAS
  • G C RAM
  • K C VERMA
  • J M KATARIA

Keywords:

Bursa of Fabricious (BF), ELISA, Infectious bursal disease virus (IBDV), Sucrose density gradient centrifugation

Abstract

A protocol to purify infectious bursal disease virus (IBDV) by source density gradient centrifugation was described. IBDV was purified from infected bursa of Fabricious (BF) of chicken by sucrose density gradient centrifugation after fluorocarbon treatment initial and pelleting. Comparison of virus material at different steps of purification by sodium dodecyl sulphate - polyacrylamide gel electrophoresis (SDS-PAGE), absorbance ratio at 260 nm and 280 nm and ELISA showed that the virus gets purified to the maximum extent only after sucrose gradient cenrifugation.

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Author Biographies

  • S C DAS
    Scientist, Division of Animal Health, Central Sheep and Wool Research Institute, Rajasthan 304 501.
  • G C RAM
    Scientist, Immunology Section, Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh 243 122

  • K C VERMA
    Scientists, Avian Diseases, Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh 243 122

  • J M KATARIA
    Scientists, Avian Diseases, Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh 243 122

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Issue

Vol. 67 No. 8 (1997)

Section

Articles

License

The copyright of the articles published in The Indian Journal of Animal Sciences is vested with the Indian Council of Agricultural Research, which reserves the right to enter into any agreement with any organization in India or abroad, for reprography, photocopying, storage and dissemination of information. The Council has no objection to using the material, provided the information is not being utilized for commercial purposes and wherever the information is being used, proper credit is given to ICAR.

How to Cite

DAS, S. C., RAM, G. C., VERMA, K. C., & KATARIA, J. M. (2013). Concentration and purification of infectious bursal disease virus (IBDV) by sucrose density gradient centrifugation. The Indian Journal of Animal Sciences, 67(8). https://epubs.icar.org.in/index.php/IJAnS/article/view/34869