Extracellular and cell-bound verocytotoxins of Salmonella serovars


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Authors

  • Y SINGH
  • V D SHARMA
  • R CHANDRA
  • S K GARG

Keywords:

Salmonella serovars, Vero cell-line, Verocytotoxicity

Abstract

The 27 strains of Salmonella belonging to 9 different serovars, namely S. Enterica sub. sp. Enterica serovar Newport, S. stanley, S. weltevreden, S. typhimurium, S. senftenberg, S. agona, S. bareilly, S. braenderup and S. kentucky isolated from foods of animal origin, were tested for their cytotoxicity on Vera cell-line. The cell sonicates of all the 27 strains of Salmonella showed variable degree of cytotoxicity, while 25 CFCS (92.5%) of these strains were found cytotoxic to Vero cells. The intensity of changes in Vero cells appeared to be dose dependent and increased irreversibly with increase in incubation period. Polymyxin B sulphate (@0.25 mglml in PBS, pH 7.2), urea (8 M in tris-HCI buffer, pH 8.2) and cell -sonication facilitated the release of cytotoxin.

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Author Biographies

  • Y SINGH
    Assistant Scientist (Senior Scale), Department of Veterinary Public Health and Epidemiology, CCS HAU, Hisar 125 004.
  • V D SHARMA
    Professor and Head, Govind Ballabh Pant University of Agriculture and Technology, Pantnagar, Uttar Pradesh 263145

  • R CHANDRA
    Associate Professor, Govind Ballabh Pant University of Agriculture and Technology, Pantnagar, Uttar Pradesh 263145
  • S K GARG
    Professor, Veterimuy Microbiology, Govind Ballabh Pant University of Agriculture and Technology, Pantnagar, Uttar Pradesh 263145

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Issue

Vol. 67 No. 8 (1997)

Section

Articles

License

The copyright of the articles published in The Indian Journal of Animal Sciences is vested with the Indian Council of Agricultural Research, which reserves the right to enter into any agreement with any organization in India or abroad, for reprography, photocopying, storage and dissemination of information. The Council has no objection to using the material, provided the information is not being utilized for commercial purposes and wherever the information is being used, proper credit is given to ICAR.

How to Cite

SINGH, Y., SHARMA, V. D., CHANDRA, R., & GARG, S. K. (2013). Extracellular and cell-bound verocytotoxins of Salmonella serovars. The Indian Journal of Animal Sciences, 67(8). https://epubs.icar.org.in/index.php/IJAnS/article/view/34874