An HPLC method for estimation of volatile fatty acids in rumimd fluid


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Authors

  • SAMUEL MATHEW
  • S SAGATHEVAN
  • J THOMAS
  • GEORGE MATHEN

Keywords:

High pressure liquid chromatography, Ruminal fluid, Volatile fatty acids

Abstract

A simple liquid chromatographic method was developed for the estimation of volatile fatty acids in ruminal fluid. The procedure involved clarification of the fluid with stannous chloride and estimation of acetic, propionic and butyric acids by HPLC using a reversed-phase column, phosphate buffer pH 2.5 eluent and UV spectrophotometric detector. The overall recovery was found to be 84.55± 7.10% for acetic acid, 70.55±0.65 % tor propionic acid and 88.46±2.98% for butyric acid. A minimum of 1.4 mM acetic acid and 0.56 mM each of propionic and butyric acids could be estimated by the method.

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Author Biographies

  • SAMUEL MATHEW

    Associate Professor, Veemanagar, Pondicherry
    605 009.
  • S SAGATHEVAN
    310dai Street, Kerala Agricultural University, Aromatic and Medicinal Plants Research Station, Odakkali, Kerala 683 549
  • J THOMAS

    Associate Professor, Veemanagar, Pondicherry
    605 009.
  • GEORGE MATHEN
    Professor, College of Veterinary and Animal Sciences, Mannuthy, Kerala 680651.

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Issue

Vol. 67 No. 9 (1997)

Section

Articles

License

The copyright of the articles published in The Indian Journal of Animal Sciences is vested with the Indian Council of Agricultural Research, which reserves the right to enter into any agreement with any organization in India or abroad, for reprography, photocopying, storage and dissemination of information. The Council has no objection to using the material, provided the information is not being utilized for commercial purposes and wherever the information is being used, proper credit is given to ICAR.

How to Cite

MATHEW, S., SAGATHEVAN, S., THOMAS, J., & MATHEN, G. (2013). An HPLC method for estimation of volatile fatty acids in rumimd fluid. The Indian Journal of Animal Sciences, 67(9). https://epubs.icar.org.in/index.php/IJAnS/article/view/35007