Adaptation of infectious bursal disease virus in chicken embryo fibroblast cell culture and its fine structure


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Authors

  • RAJESH KUMAR
  • R CHANDRA
  • V D P RAO
  • S K SHUKLA

Keywords:

Chicken embryo fibroblast cells, Electron microscopy, Infectious bursal disease, Natural outbreaks

Abstract

Two outbreaks of suspected infectious bursal disease (IBD) at university poultry farms were investigated. On the basis of
epidemiological data, characteristic gross and microscopic changes in different organs including bursa of Fabricius (BF)
and spleen, presence ofIBD virus antigen in BF and demonstration of intracytoplasmic virus particles of 59.2 nm diameter in infected chicken embryo fibroblast cells by transmission electron microscopy confirmed the outbreak as IBD. The type and distribution of lesions and mortality up to 23.2% is highly suggestive of involvement of IBD virus of moderate virulence. Neutralization of cell culture grown virus with reference antisera revealed the identity of virus as IBDV
serotype 1.


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Author Biographies

  • RAJESH KUMAR
    Senior Research Fellow,
  • R CHANDRA
    Associate Professor,
    Virology;
  • V D P RAO
    Professor, Virology, Department of Microbiology;
  • S K SHUKLA
    Associate Professor,
    Dypartment of Medicine, College of Veterinary Sciences.

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Issue

Vol. 70 No. 2 (2000)

Section

Articles

License

The copyright of the articles published in The Indian Journal of Animal Sciences is vested with the Indian Council of Agricultural Research, which reserves the right to enter into any agreement with any organization in India or abroad, for reprography, photocopying, storage and dissemination of information. The Council has no objection to using the material, provided the information is not being utilized for commercial purposes and wherever the information is being used, proper credit is given to ICAR.

How to Cite

KUMAR, R., CHANDRA, R., RAO, V. D. P., & SHUKLA, S. K. (2013). Adaptation of infectious bursal disease virus in chicken embryo fibroblast cell culture and its fine structure. The Indian Journal of Animal Sciences, 70(2). https://epubs.icar.org.in/index.php/IJAnS/article/view/35549