Yield and potency of follitropin extracted from pituitary glands of zebu cattle (Bos indicus) and water buffalo (Bubalus bubalis)


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Authors

  • A. MEHMOOD
  • M ANWAR

Keywords:

Follitropin extraction, Pituitaries, Water buffalo, Zebu cattle

Abstract

Two methods were compared for follitropin extractioll from the frozen-thawed pituitary glands of zebu cattle and water buffalo. The initial extraction was made by a gradual increase in the concentration of ammonium sulphate at different levels of pH, followed by precipitation of follitropin activity either by ethanol or by acetone. Follitropin yield(g/ kg of tissue) differed nonsignificantly (cattle: 2.6 and 3.1; buffalo: 2.4 and 2.5) between species and between methods of extraction. Biological potency of the follitropin-extract also differed nonsignificantly and tanged at 88.2%·91.7% of standard FSH.P.

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Author Biographies

  • A. MEHMOOD
    Senior Scientific Officer, ASI, NARC, Islamabad, Pakistan.
  • M ANWAR
    Senior Scientific Officer, ASI, NARC, Islamabad, Pakistan.

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Issue

Vol. 70 No. 2 (2000)

Section

Articles

License

The copyright of the articles published in The Indian Journal of Animal Sciences is vested with the Indian Council of Agricultural Research, which reserves the right to enter into any agreement with any organization in India or abroad, for reprography, photocopying, storage and dissemination of information. The Council has no objection to using the material, provided the information is not being utilized for commercial purposes and wherever the information is being used, proper credit is given to ICAR.

How to Cite

MEHMOOD, A., & ANWAR, M. (2013). Yield and potency of follitropin extracted from pituitary glands of zebu cattle (Bos indicus) and water buffalo (Bubalus bubalis). The Indian Journal of Animal Sciences, 70(2). https://epubs.icar.org.in/index.php/IJAnS/article/view/35571