Cloning of a cellulolytic gene from rumen bacteria using pUC18 vector


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Authors

  • VIJAYARANI VIJAYARANI
  • R VELMURUGAN
  • K NACHIMUTHU
  • V D PADMANABAN

Keywords:

CellUlase gene, Rumen bacteria, Ruminococcus albus

Abstract

The present work describes the cloning of a cellulolytic gene from the rumen bacteria Ruminococcus albus using pUC18 vectors. In pUC18 cloning, 3 white recombinant colonies with cellulase activity by congo red assay was obtained out of 68 colonies screened. Out of 3 colonies, 1 has a 1.4 kb Hind III insert resembling endoglucanase gene. The homology between the cloned product and genomic DNA of R. albus was checked by dot-blot hybridization. Expression of the gene was tested by growing the recombinant cells in media containing cellobiose and carboxymethyl cellulose.

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Author Biographies

  • VIJAYARANI VIJAYARANI
    Assistant Professor,
  • R VELMURUGAN
    Senior Research Fellow, Department of Animal Biotechnology,
  • K NACHIMUTHU
    Dean, Faculty of Basic
    Sciences, Madras VeterinaFY College, Chennai 600 007.
  • V D PADMANABAN
    Former Registrar, TANUVAS, 2/2 Ramaswamy Street, Chennai
    600 017.

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Issue

Vol. 70 No. 3 (2000)

Section

Articles

License

The copyright of the articles published in The Indian Journal of Animal Sciences is vested with the Indian Council of Agricultural Research, which reserves the right to enter into any agreement with any organization in India or abroad, for reprography, photocopying, storage and dissemination of information. The Council has no objection to using the material, provided the information is not being utilized for commercial purposes and wherever the information is being used, proper credit is given to ICAR.

How to Cite

VIJAYARANI, V., VELMURUGAN, R., NACHIMUTHU, K., & PADMANABAN, V. D. (2013). Cloning of a cellulolytic gene from rumen bacteria using pUC18 vector. The Indian Journal of Animal Sciences, 70(3). https://epubs.icar.org.in/index.php/IJAnS/article/view/35608