Effect of Pasteurella multocida toxin on vero cell culture


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Authors

  • JAI SUNDER
  • A A KUMAR

Keywords:

Cytopathic effect, Pasteurella multocida toxin, Vero cell

Abstract

The effect of Pasteurella multocida toxin (PMT) produced by a toxigenic strain of serotype A: 12 (goat strain) on vero cell monolayer was studied. The cytopathic effect was observed after 7 days of incubation at 37°C under 5% carbondioxide tension. The minimum cytopathic dose of tile toxin was 5.8 ug of the crude toxin. The study suggests that the cell culture (vero cells) assay can be adopted for detection and quuntitation of toxin from toxigeniC strams of P. multocida.

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Author Biographies

  • JAI SUNDER
    Ph.D Scholar, Indian Veterinary Research Institute, izatnagar, Uttar Pradesh 243 122

  • A A KUMAR
    Senior Scientist, Division of Bacteriology and Mycology.

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How to Cite

SUNDER, J., & KUMAR, A. A. (2013). Effect of Pasteurella multocida toxin on vero cell culture. The Indian Journal of Animal Sciences, 70(3). https://epubs.icar.org.in/index.php/IJAnS/article/view/35627