Humoral and cell-mediated immune responses of lambs against bluetongue virus


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Authors

  • V V DESHMUKH
  • M B GUJAR

Keywords:

BLU-1, Bluetongue virus type 1, Competitive ELISA, Complement tixation test, Cell-mediated immunity, Leucocytes migration inhibition test

Abstract

The bluetongue virus (BLU-1) was isolated from field outbreaks in Maharashtra. The Virus was adopted on BHK-21 cell lines. Humoral immune responses of lambs were evaluated by uSing competitive enzyme linked immunosorbent assay (eELISA) and complement fixation test (CFT). The cell-mediated immune response (CMI) was evaluated by using leucocyte migration inhibition test (LMIT). The cell culture adopted BLU. 1 virus was astrong immunogen. The antibody to BLU-1 was produced on seventh day post-inoculation which persisted at high level after challenge infection. The CMI was developed from seventh day post-inoculation and persisted at high level after challenge infection.

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Author Biographies

  • V V DESHMUKH
    Scientist, Animal Disease Research Centre;

  • M B GUJAR
    Professor and Head, Department of Microbiology.                       

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Issue

Vol. 70 No. 4 (2000)

Section

Articles

License

The copyright of the articles published in The Indian Journal of Animal Sciences is vested with the Indian Council of Agricultural Research, which reserves the right to enter into any agreement with any organization in India or abroad, for reprography, photocopying, storage and dissemination of information. The Council has no objection to using the material, provided the information is not being utilized for commercial purposes and wherever the information is being used, proper credit is given to ICAR.

How to Cite

DESHMUKH, V. V., & GUJAR, M. B. (2013). Humoral and cell-mediated immune responses of lambs against bluetongue virus. The Indian Journal of Animal Sciences, 70(4). https://epubs.icar.org.in/index.php/IJAnS/article/view/35726