Extraction, purification and preparation of species-specific phenolic glycolipid (PGL) from Mycobacterium bovis (AN5)


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Authors

  • SUJIT NAYAK
  • RISHENDRA VERMA
  • NIRMALA KUMARI
  • K VENKATESAN

Keywords:

Mycobacterium hovis AN5, Phenolic glycolipid (PGL)

Abstract

Phenolic glycolipid (PGL) which is species-specific to Mycobacterium bovis AN5, was extracted, purified and deaeylatcd using preparative thin fayer chromatograpflY and florisitcolumn. On single directional chromatography, using sofvent system chloroform' methanol: water (90: 10: 1, v/v x 1), the mobility of deacylated PGl of M.bovis AN5 was identica wIth the mobility of authentic deacylated PGL (Rf value 0.23).

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Author Biographies

  • SUJIT NAYAK
    Immunology Section,
  • RISHENDRA VERMA
    Division of Bactcriology and Mycology, IVRI, Izatnagur, Uttar Pradesh 243 122.
  • NIRMALA KUMARI
    Central JALMA InstItute for Leprosy, TaJ Ganj, Agra, Uttar
    Pradesh 282 001 .
  • K VENKATESAN
    Central JALMA InstItute for Leprosy, TaJ Ganj, Agra, Uttar
    Pradesh 282 001 .

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Issue

Vol. 70 No. 5 (2000)

Section

Articles

License

The copyright of the articles published in The Indian Journal of Animal Sciences is vested with the Indian Council of Agricultural Research, which reserves the right to enter into any agreement with any organization in India or abroad, for reprography, photocopying, storage and dissemination of information. The Council has no objection to using the material, provided the information is not being utilized for commercial purposes and wherever the information is being used, proper credit is given to ICAR.

How to Cite

NAYAK, S., VERMA, R., KUMARI, N., & VENKATESAN, K. (2013). Extraction, purification and preparation of species-specific phenolic glycolipid (PGL) from Mycobacterium bovis (AN5). The Indian Journal of Animal Sciences, 70(5). https://epubs.icar.org.in/index.php/IJAnS/article/view/35868