Extraction, purification and preparation of species-specific phenolic glycolipid (PGL) from Mycobacterium bovis (AN5)
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Keywords:
Mycobacterium hovis AN5, Phenolic glycolipid (PGL)Abstract
Phenolic glycolipid (PGL) which is species-specific to Mycobacterium bovis AN5, was extracted, purified and deaeylatcd using preparative thin fayer chromatograpflY and florisitcolumn. On single directional chromatography, using sofvent system chloroform' methanol: water (90: 10: 1, v/v x 1), the mobility of deacylated PGl of M.bovis AN5 was identica wIth the mobility of authentic deacylated PGL (Rf value 0.23).Downloads
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