Comparative seroreactivity of piroplasm antigens of Theileria annulata


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Authors

  • B DUTTA
  • G C BANSAL
  • D RAY
  • S K GHORUI

Keywords:

ELISA, Nonidet-P-40, Theileria annulata, Triton X-100

Abstract

Host cell free piroplasms of Theileria annulata were disrupted by repeated freezing (- 196°C) and thawing (37°C) to obtain soluble protein antigen of the parasite (fraction I). The insoluble cellular precipitates were sequentially extracted first with Triton x 100 (fraction II) and then with Nonidet P-40 (fraction III). The fraction I showed highest seroreactivity in ELISA when tested against bovine antisera with optimum concentrations of antigens and antibodies. Slab gel electrophoresis of fractionated antigens revealed negligible contamination of host cellular protein. The presence of the major immunodominant proteins of 32.5 and 97 KDa molecular weight was most conspicuous in fraction I.

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Author Biographies

  • B DUTTA
    Veterinary Surgeon, Burdwan, West Bengal.
  • G C BANSAL
    Senior Scientist, Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh 243 122

  • D RAY
    Senior Scientist,Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh 243 122

  • S K GHORUI
    Scientist, Division of Parasitology. Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh 243 122

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Issue

Vol. 70 No. 5 (2000)

Section

Articles

License

The copyright of the articles published in The Indian Journal of Animal Sciences is vested with the Indian Council of Agricultural Research, which reserves the right to enter into any agreement with any organization in India or abroad, for reprography, photocopying, storage and dissemination of information. The Council has no objection to using the material, provided the information is not being utilized for commercial purposes and wherever the information is being used, proper credit is given to ICAR.

How to Cite

DUTTA, B., BANSAL, G. C., RAY, D., & GHORUI, S. K. (2013). Comparative seroreactivity of piroplasm antigens of Theileria annulata. The Indian Journal of Animal Sciences, 70(5). https://epubs.icar.org.in/index.php/IJAnS/article/view/35870