Antigenic analysis of M. paratuberculosis strain TEPS by SDS-PAGE and two-dimensional immunoelectrophoresis


77 / 27

Authors

  • T K GOSWAMI
  • S N JOARDAR
  • G C RAM

Keywords:

Crossed-immunoelectrophoresis, Mycohacterium paratuberculosis, Mycobacterial antigens, SDS-PAGE

Abstract

Mycobacterial antigens are usually complex in nature. Immunoelectrophoretic technique may not be efficient in separation of such complex antigens owing to poor resolving efficacy. In this report, protein components of Ai. paratuberculosis were resolved into 40 polypeptide fractions by SDS-PAGE as detected by Coommassie brilliant blue staining whereas only 8 bands were detected by periodic acid schiff's staining. On crossed immunoelectrophoresis, sonicate an tigen of M. paratuberculosis with hyperimmune goat sera has shown more than 18 immunogenic precipitation lines suggestive of their immunodominant nature.

Downloads

Download data is not yet available.

Author Biographies

  • T K GOSWAMI
    Scientist,
  • S N JOARDAR
    Assistant Professor, Department of Fishery Microbiology and Pathology, West Bengal University of Animal and Fishery Sciences, Calcutta 700 037.
  • G C RAM
    Scientist In-charge, Immunology Section.

Downloads

Issue

Vol. 70 No. 8 (2000)

Section

Articles

License

The copyright of the articles published in The Indian Journal of Animal Sciences is vested with the Indian Council of Agricultural Research, which reserves the right to enter into any agreement with any organization in India or abroad, for reprography, photocopying, storage and dissemination of information. The Council has no objection to using the material, provided the information is not being utilized for commercial purposes and wherever the information is being used, proper credit is given to ICAR.

How to Cite

GOSWAMI, T. K., JOARDAR, S. N., & RAM, G. C. (2014). Antigenic analysis of M. paratuberculosis strain TEPS by SDS-PAGE and two-dimensional immunoelectrophoresis. The Indian Journal of Animal Sciences, 70(8). https://epubs.icar.org.in/index.php/IJAnS/article/view/36152