Kinetoplast minicircle DNA analysis of Trypanosoma evansi by polymerase chain reaction using single oligonucleotide primers


106 / 16

Authors

  • S OMANWAR
  • J R RAO
  • R K SINGH

Keywords:

Arbitrary primer, Kinetoplast DNA, PCR, Trypanosoma evansi

Abstract

Kinetoplast DNA from Trypanosoma evqnsi isolates of bubaline, equine and cameline origin were amplified by polymerase cham. reaction (PCR) using 4 arbitrarily, selected 10 to 11 base- primers. Depending upon the T evansi isolate-primer combmation, between 3 to 8 reproducible DNA fingerprints of 300 to 1630 bp were amplified revealing a limited DNA polymorphism.

Downloads

Download data is not yet available.

Author Biographies

  • S OMANWAR
    House 2090, Phase X, Mohali Chandigarh 160 022
  • J R RAO
    Senior Scientist, Division of Parasitology,
  • R K SINGH
    Scientist (Senior scale National Biotechnology Centre

Downloads

Issue

Vol. 71 No. 1 (2001)

Section

Articles

License

The copyright of the articles published in The Indian Journal of Animal Sciences is vested with the Indian Council of Agricultural Research, which reserves the right to enter into any agreement with any organization in India or abroad, for reprography, photocopying, storage and dissemination of information. The Council has no objection to using the material, provided the information is not being utilized for commercial purposes and wherever the information is being used, proper credit is given to ICAR.

How to Cite

OMANWAR, S., RAO, J. R., & SINGH, R. K. (2014). Kinetoplast minicircle DNA analysis of Trypanosoma evansi by polymerase chain reaction using single oligonucleotide primers. The Indian Journal of Animal Sciences, 71(1). https://epubs.icar.org.in/index.php/IJAnS/article/view/36331