Comparison of protein profile of Newcastle disease virus isolated from different avian species


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Authors

  • S MISHRA
  • J M KATARIA
  • K C VERMA
  • J P MISHRA

Keywords:

Chicken, Guinea fowl, Newcastle disease virus, Pigeon, Protein profile, Western blotting

Abstract

The Newcastle disease viruses isolated from chicken, guinea fowl and pigeon were purified by sucrose density gradient centrifugation at 80 000 rpm for I hr. The protein profile of the virus isolates was compared by subjecting the purified
preparation of viruses to SDS-PAGE, Electrophoretic migration pattern of protein of all the isolates revealed 7 protem bands on gel according to their molecular weight and no variation was observed among them. Immunogenic proteins of the isolates were compared by transferring the proteins to membrane and then by immunoblotting using homologoLis and heterologous sera. The three isolates reacted evenly with the sera showing no difference at their immunogenic proteins.

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Author Biographies

  • S MISHRA
    Scientist, CSWRI, Avikanagar, Rajasthan 304501,
  • J M KATARIA
    Senior Scientist,
  • K C VERMA
    Principal Scientist and Head, Division of Avian Diseases,
  • J P MISHRA
    Scientist, CSWRI, Avikanagar, Rajasthan 304501,

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Issue

Vol. 71 No. 2 (2001)

Section

Articles

License

The copyright of the articles published in The Indian Journal of Animal Sciences is vested with the Indian Council of Agricultural Research, which reserves the right to enter into any agreement with any organization in India or abroad, for reprography, photocopying, storage and dissemination of information. The Council has no objection to using the material, provided the information is not being utilized for commercial purposes and wherever the information is being used, proper credit is given to ICAR.

How to Cite

MISHRA, S., KATARIA, J. M., VERMA, K. C., & MISHRA, J. P. (2014). Comparison of protein profile of Newcastle disease virus isolated from different avian species. The Indian Journal of Animal Sciences, 71(2). https://epubs.icar.org.in/index.php/IJAnS/article/view/36401