A comparative study of outer membrane proteins of selected Brucella strains


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Authors

  • S ISLOOR
  • V V S SURYANARAYANA
  • M SATYANARAYANA RAO
  • M RAJASEKHAR

Keywords:

Brucella, Outer membrane proteins, SDS-PAGE

Abstract

Outer membrane proteins (OMPs) of Brucella abortus strains (99,544 and 19), Brucella melitensis 16M and Brucella suis 1330 were isolated by differential centrifugation method, Based on sodium dodecyl sulphate - polyacrylamide gel electrophoresis (SDS-PAGE) profiles, Brucella OMPs were classified into 3 distinct molecular weight (MW) ranges, viz, low MW (14-30 kDa), moderate MW (39-60 kDa) and high MW (68-93 kDa), Polypeptides above 60 kDa were not observed in B, abortus 544. The moderate MW bands were more varied in their migration and relative quantity, Silver staining confirmed the presence of polysaccharide moieties in major bands at position 39,2-47,3 kDa,The subtle differences observed in SDS-PAGE analysis of Brucella OMPs did not represent rei iable marker for identification of Brucella species.

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Author Biographies

  • S ISLOOR
    Scientist, Project Directorate on Animal Disease Monitoring and Surveillance (PD-ADMAS), Hebbal, Bangalore, Karnataka 560 024 India
  • V V S SURYANARAYANA
    Principal Scientist, IVRl, Izatnagar, Uttar Pradesh 243 122,
  • M SATYANARAYANA RAO
    Professor, Department of Public Health, Veterinary College,
    UAS, Hebbal, Bangalore, Karnataka 560 024 .
  • M RAJASEKHAR
    Project Directorate on Animal Disease Monitoring and Surveillance (PD-ADMAS), Hebbal, Bangalore, Karnataka 560 024 India

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Issue

Vol. 71 No. 3 (2001)

Section

Articles

License

The copyright of the articles published in The Indian Journal of Animal Sciences is vested with the Indian Council of Agricultural Research, which reserves the right to enter into any agreement with any organization in India or abroad, for reprography, photocopying, storage and dissemination of information. The Council has no objection to using the material, provided the information is not being utilized for commercial purposes and wherever the information is being used, proper credit is given to ICAR.

How to Cite

ISLOOR, S., SURYANARAYANA, V. V. S., RAO, M. S., & RAJASEKHAR, M. (2014). A comparative study of outer membrane proteins of selected Brucella strains. The Indian Journal of Animal Sciences, 71(3). https://epubs.icar.org.in/index.php/IJAnS/article/view/36470