Preparation of eye balls of rat and mice for histopathological examination in toxicological-studies

Abstract

A study was conducted to standardize the procedure for preparation of eye balls of rats and mice, for histopathological
evaluation in toxicological studies, using 10% n-eutral buffered formalin and Davidson's fluid. Davidson's fluid proved to . I be a good fixative, but prolonged' fixation in 'the same, hardened the lenses. Formalin fixation hardened the lenses to a greater extent and thus resulted in artefactual changes. Better results were obtained with chloroform, as a clearing agent, when compared to xylene. More than 95% results were attained with shorter duration of fixation in Davidson's fluid, along with use of chloroform as a clearing agent during processing.