Embryo sexing with heterochromatin staining technique


Abstract views: 126 / PDF downloads: 15

Authors

  • B R YADAV
  • W A KING
  • K P XU
  • J W POLLARD
  • K J BETTERJDGE

Keywords:

C-banding, Chromosome, Embryo, Heterochromatin staining, Sex diagnosis

Abstract

The embryos were produced in vitro for the study of their development and sex identification by sex chromosomes (cytogenetical method). The embryos were examined to identify the x- and Y-chromosomes and thus sex of the embryos. Conventionally stained embryos were examined for the location of metaphase plates and identification of sex chromosomes. Subsequently the embryos revealing metaphase plates were C-banded to further increase the sex diagnosis. Among the fixed embryos 936/1251 (74.92%) showed metaphascs. In conventional staining 524/936 (55.98%) embryos could be sexed which upon heterochromatin staining revealed 695/936 (74.26%). Chromosome analysis of conventional staining in conjunction with heterochromatin staining is very accurate technique for sex diagnosis in preimplantation embryos at various stages of development from cleavage to hatching besides confirmation of sex it can also be used for other chromosome anomalies afflicting development, growth reproduction etc. later in life.

Downloads

Download data is not yet available.

Author Biographies

  • B R YADAV
    National Fellow, Livestock Genome Analysis
    Laboratory, Dairy Cattle Breeding Division, National Dairy Research Institute, Karnal 132 001 India.
  • W A KING
    Professor, University of Guelph, Guelph, NIG, 2WI

  • K P XU
    PDF, University of Guelph, Guelph, NIG, 2WI
  • J W POLLARD
    Ph.D.Scholar, University of Guelph, Guelph, NIG, 2WI
  • K J BETTERJDGE
    Professor and Head, Animal Biotechnology and Embryo Laboratory, Department of Biomedical Sciences, Ontario Veterinary College, Ontario, Canada.

Downloads

Issue

Vol. 71 No. 5 (2001)

Section

Articles

License

The copyright of the articles published in The Indian Journal of Animal Sciences is vested with the Indian Council of Agricultural Research, which reserves the right to enter into any agreement with any organization in India or abroad, for reprography, photocopying, storage and dissemination of information. The Council has no objection to using the material, provided the information is not being utilized for commercial purposes and wherever the information is being used, proper credit is given to ICAR.

How to Cite

YADAV, B. R., KING, W. A., XU, K. P., POLLARD, J. W., & BETTERJDGE, K. J. (2014). Embryo sexing with heterochromatin staining technique. The Indian Journal of Animal Sciences, 71(5). https://epubs.icar.org.in/index.php/IJAnS/article/view/36654