Effect of follicular fluid on acrosomal integrity of buffalo spermatozoa*

Abstract

This study was conducted to evaluate the effect of bub aline follicular fluid (FF) on aero somal integrity of buffalo spermatozoa. Steroid free and whole FF from buffalo ovaries at the concentration of I % alone and in combination with cysteine HCI (0.1 %) and EDT A (0.1 %) were used as additive in tris dilutor for preservation of washed and unwashed buffalo spermatozoa at 4°C and -196°C. The intact acrosome and abnormal acrosome at different stages of preservation were assessed. The effect of FF on acrosome was significant (P<0.01), and inclusion of FF as additive in tris dilutor exerted a protective effect on the acrosome during refrigerated preservation and freezing. This is an indicative of some synergistic action of FF along with other cryoprotectants in the dilutor.