Effect of platelet activating factor on some seminal attributes in crossbred cattle


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Authors

  • SUBODH KUMAR
  • ARJAVA SHARMA

Keywords:

Abnormalities, Acrosome reaction, Cattle, Live count, Motility, Platelet activating factor, Seminal attributes, Spermatozoa

Abstract

Frozen semen samples of 12 crossbred cattle (Karan Fries) bulls with varying conception rates were taken and after appropriate thawing and washing in modified sperm T ALP medium, they were treated with 200 mM platelet activating factor (PAF) for 15 min at 38°C and 5% Coz leyel. Comparison was made with controls (not PAF treated) for motility, acrosome reaction (AR), live count (LC), head (HD-ABN), mid piece (MP-ABN), tail (TL-ABN) and total (TTL-ABN) abnormalities. PAF caused increase in AR, HD-ABN, TL-ABN, TTL-ABN, decrease in motility, LC, whereas, it did not affect MP-ABN. The paired t-test revealed that effect of PAF on all the parameters except MP-ABN was significant.

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Author Biographies

  • SUBODH KUMAR
    Scientist, NRC-Mithun, Jharnapani, Medziphema, Nagaland 797 106.
  • ARJAVA SHARMA
    Principal Scientist, DCB Division, NDRI, Karnal, Haryana 132 001.

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How to Cite

KUMAR, S., & SHARMA, A. (2014). Effect of platelet activating factor on some seminal attributes in crossbred cattle. The Indian Journal of Animal Sciences, 71(10). https://epubs.icar.org.in/index.php/IJAnS/article/view/37017