Optimal conditions for assay of haemolytic component of camel complement in classical pathway


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Authors

  • V CHOPRA
  • S K KASHYAP

Keywords:

Camel, Camel complement, Classical pathway, Haemolytic component

Abstract

Haemolytic complement of camel serum was assayed under standardized conditions. CH50 titres in the range of 110.03-417.8 units ml-1 of camel serum with a mean of 197.8 were obtained. Effect of various variables on the lytic activity of camel complement in classical pathway was studied. The standard system of titrating the HC level consisted of rabbit erythrocytes sensitized with goat haemolysin as indicator system, veronal buffer saline, pH 7.3, ionic strength 0.15M, Ca2+ and Mg2+ concentrations of 4.10-4 and 1.10-3M, respectively. Incubation at 37°C for 60 min gave the highest HC activity. Higher Na2 EDT A concentrations than 5.10-4 M inhibited haemolytic activity to undetectable levels.

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Author Biographies

  • V CHOPRA
    Veterinary Officer, Veterinary Hospital, Suhlai, Bikaner 334 007.
  • S K KASHYAP
    Head, Department of Veterinary Microbiology, College of
    Veterinary and Animal Science.

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Issue

Vol. 71 No. 11 (2001)

Section

Articles

License

The copyright of the articles published in The Indian Journal of Animal Sciences is vested with the Indian Council of Agricultural Research, which reserves the right to enter into any agreement with any organization in India or abroad, for reprography, photocopying, storage and dissemination of information. The Council has no objection to using the material, provided the information is not being utilized for commercial purposes and wherever the information is being used, proper credit is given to ICAR.

How to Cite

CHOPRA, V., & KASHYAP, S. K. (2014). Optimal conditions for assay of haemolytic component of camel complement in classical pathway. The Indian Journal of Animal Sciences, 71(11). https://epubs.icar.org.in/index.php/IJAnS/article/view/37198