Determination of optimal in vitro conditions for camel alternative complement pathway assay


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Authors

  • V CHOPRA
  • S K KASHYAP

Keywords:

Alternate pathway, Camel complement

Abstract

Optimal in vitro testing conditions for camel,alternative complement pathway assay were determined. Effect of following variables were tested: heterologous erythrocytes, pH, ionic strength and Mg2+ ion concentration of diluent, incubation time and temperature. The standard system of titrating the He levels consisted of rabbit erythrocytes as target cells, veronal buffer saline with EGTA, pH 7.3, ionic strength 0.10 M, Mg2+ concentration 4mM at 37°C temperature for 60 min incubation.

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Author Biographies

  • V CHOPRA
    Veterinary Officer, Sublai, Bikaner 334 007.
  • S K KASHYAP
    Head, Department of Veterniary Microbiology, College of
    Veterinary and Animal Sciences.

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Issue

Vol. 71 No. 11 (2001)

Section

Articles

License

The copyright of the articles published in The Indian Journal of Animal Sciences is vested with the Indian Council of Agricultural Research, which reserves the right to enter into any agreement with any organization in India or abroad, for reprography, photocopying, storage and dissemination of information. The Council has no objection to using the material, provided the information is not being utilized for commercial purposes and wherever the information is being used, proper credit is given to ICAR.

How to Cite

CHOPRA, V., & KASHYAP, S. K. (2014). Determination of optimal in vitro conditions for camel alternative complement pathway assay. The Indian Journal of Animal Sciences, 71(11). https://epubs.icar.org.in/index.php/IJAnS/article/view/37199