Salivary gland tannins binding proteins of sheep and goat

Abstract

Trichloroacetic acid soluble proteins (TCASP) of sheep and goats were isolated. The ratios of parotid gland weight to body weight were 0.839±0.061 and 1.293±0.121, respectively, for sheep and goats. The TCASP in mg/g tissue were 6.02±0.15 and 6.41±0.34 and in mg/kg body weight were 5.06±0.47 and 8.24±0.64, respectively, for sheep and goats. But TCASP of both the species had absorption maximilm at 260.6nm. Relative binding capacity of TCASP towards condensed tannins from Zizyphus nummularia and Prosopis cilleraria were 2.94 and 1.67; 1.33 and 1.11, respectively, for sheep and goats. SDS-PAGE analysis ofTCASP of sheep and goats before and after binding with condensed tannins indicated that the polypeptides precipitated with tannins were of 76 and 68 KDa in sheep and of 74 and 68 KDa in goats. The polypeptides which did not precipitate with tannins were different in both the species.