Detection of rabies virus genome in brain tissues by using in situ hybridization


125 / 26

Authors

  • CH CHAKARAVARTHY REDDY
  • R JAYAKUMAR
  • K KUMANAN
  • A MAGALINGA NAINAR

Keywords:

Brain tissue, Genome, Rabies virus

Abstract

Rabies virus is usually demonstrated in brain tissues by antigen detection. In the present study an attempt was made to detect rabies RNA in paraffin embedded tissues using in situ hybridization. Brain samples (69) from rabies suspected
animals and 15 brain samples from experimentally inoculated mice (with street rabies strain) were screened first by fluorescent antibody test (FAT) and then by in situ bybridization (ISH) using digoxigenin labelled nucleic acid probes compared with the standard FAT. ISH gave good cellular localization signals in positive slides. ISH has potential ability in studying rabies pathogenesis and in gene expression studies.

Downloads

Download data is not yet available.

Author Biographies

  • CH CHAKARAVARTHY REDDY
    Veterinary Assistant Surgeon, Department of Animal Husbandry, Andhra Pradesh.
  • R JAYAKUMAR
    Professor, Tamil Nadu Veterinary and Animal Sciences University, Chennai, Tamil Nadu 600 007 India

  • K KUMANAN
    Associate Professor,Rabies Unit, Tamil Nadu Veterinary and Animal Sciences University, Chennai, Tamil Nadu 600 007 India

  • A MAGALINGA NAINAR
    Professor and Head, Department of Animal Biotechnology, Madras Veterinary College, Chennai 600 007.

Downloads

Issue

Vol. 72 No. 1 (2002)

Section

Articles

License

The copyright of the articles published in The Indian Journal of Animal Sciences is vested with the Indian Council of Agricultural Research, which reserves the right to enter into any agreement with any organization in India or abroad, for reprography, photocopying, storage and dissemination of information. The Council has no objection to using the material, provided the information is not being utilized for commercial purposes and wherever the information is being used, proper credit is given to ICAR.

How to Cite

REDDY, C. C., JAYAKUMAR, R., KUMANAN, K., & NAINAR, A. M. (2014). Detection of rabies virus genome in brain tissues by using in situ hybridization. The Indian Journal of Animal Sciences, 72(1). https://epubs.icar.org.in/index.php/IJAnS/article/view/37345