Adaptation of R2B strain of Newcastle disease virus in vero cells


134 / 32

Authors

  • NAMITA JOSHI
  • S K GARG
  • RAJESH CHANDRA
  • V D P RAO

Keywords:

Adaptation in vero cells, Newcastle disease, Newcastle disease virus

Abstract

In quest of a cell culture adapted vaccine strain for Newcastle disease (ND), the R2B strain virus (NDV) was propagated in vero cells. The growth and adaptation of virus were assessed by haemagglutination (HA) and infectivity titres and by study of cytopathic effects (CPE). The virus adapted well to vero cells and produced characteristic cytopathic effects like formation of syncytia, stellate cells, cytoplasmic vacuolation and intracytoplasmic inclusion bodies. With the increase in passage level, the infectivity titre of virud increased 'gradually, but the HA titre decreased. The pathogenicity indices revealed reduction in the virulence of virus after adaptation to vero cells.

Downloads

Download data is not yet available.

Author Biographies

  • NAMITA JOSHI
    Assistant Professor, College of Veterinary
    Sciences, Kumarganj.
  • S K GARG
    Professor, Govind Ballabh Pant University of Agriculture and Technology, Pantnagar, Uttaranchal 263 145 India

  • RAJESH CHANDRA
    Associate Professor, Department of Veterinary
    Microbiology. College of Veterinary Sciences.
  • V D P RAO
    Professor and Head, Department of Microbiology, College of
    Veterinary Science, Tirupati.

Downloads

Issue

Vol. 72 No. 3 (2002)

Section

Articles

License

The copyright of the articles published in The Indian Journal of Animal Sciences is vested with the Indian Council of Agricultural Research, which reserves the right to enter into any agreement with any organization in India or abroad, for reprography, photocopying, storage and dissemination of information. The Council has no objection to using the material, provided the information is not being utilized for commercial purposes and wherever the information is being used, proper credit is given to ICAR.

How to Cite

JOSHI, N., GARG, S. K., CHANDRA, R., & RAO, V. D. P. (2014). Adaptation of R2B strain of Newcastle disease virus in vero cells. The Indian Journal of Animal Sciences, 72(3). https://epubs.icar.org.in/index.php/IJAnS/article/view/37467