Development of in vitro produced bovine embryos in four different culture media


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Authors

  • IBRAHIM DOGAN
  • HAKAN SAGIRKAYA
  • M KEMAL SOYLU
  • ZEKARIYA NUR
  • HUSEYIN YERLIKAYA

Keywords:

Bovine, Embryo, Fertilization

Abstract

The objective of this study was to compare the developmentai ability of embryos in 4 different media, CR I aa, modified CR2, BCEM-g and TCM-199. Bovine oocytes were matured and then fertilized in vitro. A total of 915 oocytes = were used for fertilizatiqn, 681 (74%) of which cleaved, The 2-cell embryos were divided into 4 groups, and cultured in 1 of the 4 different culture media. Percentage of development to morula-blastocyst stage from the 2 cell stage were determined as 46. 31,30 and 28% for CRI aa, CR2, BCEM-g and TCM-I99 culture media respectively. The development rate obtained with CR I aa was significantly'higher (P<0.01) compared to that with the other media.

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Author Biographies

  • IBRAHIM DOGAN
    Department of Reproduction and Artificial
    Insemination, Faculty of Veterinary Medicine.
  • HAKAN SAGIRKAYA
    Department of Reproduction and Artificial
    Insemination, Faculty of Veterinary Medicine.
  • M KEMAL SOYLU
    Department of Reproduction and Artificial
    Insemination, Faculty of Veterinary Medicine.
  • ZEKARIYA NUR
    Department of Reproduction and Artificial
    Insemination, Faculty of Veterinary Medicine.
  • HUSEYIN YERLIKAYA
    Department of Reproduction and Artificial
    Insemination, Faculty of Veterinary Medicine.

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Issue

Vol. 72 No. 3 (2002)

Section

Articles

License

The copyright of the articles published in The Indian Journal of Animal Sciences is vested with the Indian Council of Agricultural Research, which reserves the right to enter into any agreement with any organization in India or abroad, for reprography, photocopying, storage and dissemination of information. The Council has no objection to using the material, provided the information is not being utilized for commercial purposes and wherever the information is being used, proper credit is given to ICAR.

How to Cite

DOGAN, I., SAGIRKAYA, H., SOYLU, M. K., NUR, Z., & YERLIKAYA, H. (2014). Development of in vitro produced bovine embryos in four different culture media. The Indian Journal of Animal Sciences, 72(3). https://epubs.icar.org.in/index.php/IJAnS/article/view/37478