Isolation and purification of outer membrane proteins of Pasteurella multocida

Abstract

Outer membrane vesicles were prepared by extracting bacterial pellet with LCA buffer at pH 6.0. Fractionation of outer membrane vesicles on Sepharose 6 B- 100 colurhn yielded 3 peaks. Protein profile on 11 % SDS -polyacrylamide gel demonstrated that peak 1 contained outer membrane proteins of 32 kD, 40 kD, 42.5 kD, 52.5 kD, 62.5 kD and 66 kD molecular weight. of these 32 kD OMP is a marker protein for Asian HS isolates and has a definite role in protection against Pasteurella multocida infection.