Detection of BoLA-DRB3 gene polymorphism in Gir and Kankrej cattle using PCR-RFLP


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Authors

  • C P ACHARYA
  • D L PIPALIA
  • D N RANK
  • C G JOSHI
  • J V SOLANKI
  • R R SHAH

Keywords:

BoLA-DRB3, Cattle, Gene, Polymorphism, PCR-RFLP

Abstract

BoLA-DRB3 gene polymorphism was investigated in 2 cattle breeds, Gir and Kankrej by PCR-RFLP. Genomic DNA was isolated from blood samples collected from 57 Gir and 51 Kankrej animals. A 284 bp BoLA - DRB3 exon2 segment was amplified by hemi-nesting PCR, using the locus specific primers and the PCR products were subjected to Hae III and Rsa 1 digestion. Digestion with Hae III resulted in 3 RFLP patterns a, band din 5 different combinations (aa, ab, ad, bb and bd) in Gir, and 3 RFLP patterns a, band e in 4 different combinations (aa, ab, ae and be) in KankreJ. Pattern a and b were more frequent in both the breeds. Rsa 1 restriction patterns were very complex. The fragment interpretations evealed 13 different restriction patterns with 25 combinations in Gir and 10 different combinations in Kankrej. Thus, BoLA DRB3 was highly polymorphic.

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Author Biographies

  • C P ACHARYA
    M.Y.Sc. Scholar, Gujarat Agricultural University, Anand, Gujarat 388 001 India

  • D L PIPALIA
    Senior Research Fellow, Gujarat Agricultural University, Anand, Gujarat 388 001 India
  • D N RANK
    Assistant Research Scientist, Gujarat Agricultural University, Anand, Gujarat 388 001 India
  • C G JOSHI
    Assistant Research Scientist, Gujarat Agricultural University, Anand, Gujarat 388 001 India
  • J V SOLANKI
    Professor and Head, Department of Animal Genetics and Breeding, College of veterinary Science and Animal Husbandry, Anand 388 001
  • R R SHAH
    Professor and Head, Department of Animal Genetics and Breeding, College of veterinary Science and Animal Husbandry, Sardar Krushinagar 385 506.

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Issue

Vol. 72 No. 8 (2002)

Section

Articles

License

The copyright of the articles published in The Indian Journal of Animal Sciences is vested with the Indian Council of Agricultural Research, which reserves the right to enter into any agreement with any organization in India or abroad, for reprography, photocopying, storage and dissemination of information. The Council has no objection to using the material, provided the information is not being utilized for commercial purposes and wherever the information is being used, proper credit is given to ICAR.

How to Cite

ACHARYA, C. P., PIPALIA, D. L., RANK, D. N., JOSHI, C. G., SOLANKI, J. V., & SHAH, R. R. (2014). Detection of BoLA-DRB3 gene polymorphism in Gir and Kankrej cattle using PCR-RFLP. The Indian Journal of Animal Sciences, 72(8). https://epubs.icar.org.in/index.php/IJAnS/article/view/37707