Purification and characterization of Fasciola gigantica antigens by anion exchange chromatography

Abstract

Anion exchange chromatography was attempted to isolate immunodiagnostic protein moieties from crude extracts ofFasciola gigantica. The somatic antigen was fractionated into 100 fractions of which 17 fractions collected by O. J M, 0.15M and a.2M NaCI gave strong reaction in dot ELISA using anti F gigantica cattle sera raised experimentally. Sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) of intensely reacted fractions resolved 13 polypeptides in the range of 86.3-11.8 leOA in fraction 4th, 10 polypeptides of 11.8 to 86.3 KDa in fraction 14 and 12 polypeptides of 97.4 to 11.8 kOa in fraction 21 st. Of the different polypeptides, one protein band of 86.3 kOa was strongly reactive in all the fractions when tested by EITB. The purified antigens showed promising results for the early diagnosis of fasciolosis