Escherichia coli isolates from domestic poultry


144 / 60

Authors

  • ARCHANA MISHRA
  • RAKESH SHARDA
  • DAUEET CHHABRA
  • M. N MOGHE

Keywords:

Congo-red, Enterotoxin, Escherichia coli, Haemolysis, Poultry, Pathogeneclty, Vaccine

Abstract

Strains (50) of Escherichia coli were isolated from 250 clinical specimens collected from poultry birds: majority of these were recovered from heart blood. Biochemical characterisation revealed 48.0 and 16.0% atypical strains producing H2S and urease respectively. Isolates (43) were typed into 17 different 'O' serotypes; 078 was the predominant. The other serogroups isolated in decreasing frequency were O2,O19,O20, O90,O38, 0109,O81,O148, O162,08,016,O60, O63, O68, O139 and O152, some of which are of zoonotic significance. Six strains remained untypable and 1 was rough. All the isolates were Congo-red (CR)-positive  and 15 produced haemolysis on sheep blood agar. Whole bacterial culture of 45 strains and cell free flitrates of all the isolates caused mortality in mice and chicken embryos. Serotypes 08 and 078 produced enterotoxin in the ligated rabbit ileal loops. A polyvalent adjuvanted vaccine was prepared and found immunogenic.

Downloads

Download data is not yet available.

Author Biographies

  • ARCHANA MISHRA
    Veterinary Assistant Surgeon, Durg, Chattisgarh 491 001.
  • RAKESH SHARDA
    Section Head, Indira Gandhi University of Agriculture and Technology, Mhow, Madhya Pradesh 453 446 India
  • DAUEET CHHABRA
    Assistant Professor,  Indira Gandhi University of Agriculture and Technology, Mhow, Madhya Pradesh 453 446 India
  • M. N MOGHE
    Formerly Assistant Professor, Department of Microbiology, College of Veterinary Science.

Downloads

Issue

Vol. 72 No. 9 (2002)

Section

Articles

License

The copyright of the articles published in The Indian Journal of Animal Sciences is vested with the Indian Council of Agricultural Research, which reserves the right to enter into any agreement with any organization in India or abroad, for reprography, photocopying, storage and dissemination of information. The Council has no objection to using the material, provided the information is not being utilized for commercial purposes and wherever the information is being used, proper credit is given to ICAR.

How to Cite

MISHRA, A., SHARDA, R., CHHABRA, D., & MOGHE, M. N. (2014). Escherichia coli isolates from domestic poultry. The Indian Journal of Animal Sciences, 72(9). https://epubs.icar.org.in/index.php/IJAnS/article/view/37748