Detection of canine parvovirus (CPV) DNA by polymerase chain reaction assay and its prevalence in dogs in and around Kolkata, West Bengal

Abstract

The early detection of the canine parvo virus (CPV) is of paramount importance. Canine faecal samples from 306 dogs suffering from gastroenteritis in and around Kolkata were collected, and DNA was extracted by phenol-chloroform method. CPY vaccine strain was used as a positive control for CPY. Polymerase chain reaction (PCR) was carried out to amplify VPI/YP2 gene using a set of 19-mer primers [CPV - PI (Forward): 5'- ATG GCA CCT CCG GCA AAG A-3'; CPY - P, (Reverse): 5'-TTT CTA GGT GCT AGT TGA G3')]. A peR product of approximately 2.2 kb was generated with positive faecal samples and vaccine strain CPY vims. After screening, 103 dogs were found positive for CPV, but no sex variation was noted amongst the CPV positive cases. Dogs, ofthe age group of0-6 months were mostly susceptible with highest mortality rate followed by 6-12 months and 12 and above months of age and highest occurrence was noted during summer followed by rainy season and winter