Effect of blood transfusion and ringer's lactate infusion on disposition kinetics and dosage regimen of oxytetracycline in anaemic dogs

Abstract

The present study was conducted on 18 anaemic dogs divided into 3 groups of 6 animals each. The group-1(G 1) was kept as control group. In the animals of group 2 (G2), blood transfusion was undertaken @ 15 ml kg-I body weight(wt) with fresh blood collected from healthy donor dogs. In group 3 (G3), ringer's lactate was administered@ 15 ml kg-1 body
wt. The disposition kinetics of oxytetracycline (OTC) following single intramuscular administration @ 7 mg kg-1 body wt was determined in all the 3 groups. In all groups, the drug was rapidly absorbed into systemic circulation. At all time intervals, the plasma OTC levels were higher in G3 followed by G1 and 2. The absorption half-life was similar in Gland 2, but reduced in G3. However, the elimination half-life of OTC was markedly increased following infusion of blood and ringer's lactate in comparison to control group. Based on the disposition kinetic parameters and taking mininmum inhibitory concentration (MIC) as 1.0 µg-1 the convenient and suitable dosage regimen of OTC was computed in the 3 groups. In GI dogs, the priming dose (D) was calculated to be 13.0 mg kg-1 body wt followed by a maintenance does of 11.0 mg kg-1 followed by 10.0 mg kg at 12 hr inhervals in recommended.