Estimation of genetic relatedness of naked neck and normally feathered broilers using randomly amplified polymorphic DNA markers*


198 / 19

Authors

  • B N PATRA
  • R K S BAIS
  • DEEPAK SHARMA
  • R B PRASAD
  • B P SINGH

Keywords:

Broilers, DNA, Genetic relatedness, Genetic distance, Genetic similarity, RAPD-PCR

Abstract

Randomly amplified polymorphic DNA (RAPD) markers were used to analyse the genetic polymorphism between naked neck and normally feathered broilers and this polymorphism was further utilized in estimating the genetic similarity/genetic distance between these populations. Five of the 9 random primers (about 56%) revealed reproducible-polymorphic
pattern and 2 primer revealed monomorphic pattern. A total or 42 bands scored in which 12 bands (28.57%) were polymorphic. The results revealed that the genetic groups had high genetic similarity between themselves. Among all the
possible comparison, the white plumage heterozygous naked neck (WPNa/na) showed more genetic similarity (0.975)
with colour plumage heterozygous naked neck (CPNa/na) broiler. The next closer relationship (0.945) was found among
white plumage normally feathered and coloured plumage normally feathered broilers. The genetic distance based on band sharing was lowest (0.025) among WPNa/na and CPNa/na followed by WPna/na and CPna/na (0.055). The highest genetic distance was between WPNa/na and CPna/na (0.163). The present study revealed the use of RAPD markers in detecting the polymorphism and genetic relatedness of naked neck and normally feathered broilers.

Downloads

Download data is not yet available.

Author Biographies

  • B N PATRA
    Ph.D. Scholar, Division of Animal Genetics, IVRI, Izatnagar 243 122.
  • R K S BAIS
    Associate Professor, Poultry Breeding, Gavind Ballabh Pant University of Agriculture and Technology, Pantnagar, Uttaranchal 263 145 India


  • DEEPAK SHARMA
    Head, Department of Avian Genetics and Breeding, Central
    Avian Research Institute, Izatnagar 243 122 Uttar Pradesh.
  • R B PRASAD
    Head, Department of Genetics, Gavind Ballabh Pant University of Agriculture and Technology, Pantnagar, Uttaranchal 263 145 India


  • B P SINGH
    National Fellow, Gavind Ballabh Pant University of Agriculture and Technology, Pantnagar, Uttaranchal 263 145 India


Downloads

Issue

Vol. 74 No. 1 (2004)

Section

Articles

License

The copyright of the articles published in The Indian Journal of Animal Sciences is vested with the Indian Council of Agricultural Research, which reserves the right to enter into any agreement with any organization in India or abroad, for reprography, photocopying, storage and dissemination of information. The Council has no objection to using the material, provided the information is not being utilized for commercial purposes and wherever the information is being used, proper credit is given to ICAR.

How to Cite

PATRA, B. N., BAIS, R. K. S., SHARMA, D., PRASAD, R. B., & SINGH, B. P. (2014). Estimation of genetic relatedness of naked neck and normally feathered broilers using randomly amplified polymorphic DNA markers*. The Indian Journal of Animal Sciences, 74(1). https://epubs.icar.org.in/index.php/IJAnS/article/view/38234