Molecular characterization of Pasteurella multocida isolates from baemorrbagic septicaemia by ribotyping


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Authors

  • A K ARORA
  • S K JAND
  • K S SANDHU
  • M S OBEROI

Keywords:

Haemorrhagic septicaemia, Pasteurella rnultocida, Restriction endonuclease analysis, Ribotyping

Abstract

Characterization of 17 isolates of Pasteurella multocida (serotype B:2) from haemorrhagic septicaemia in cattle and buffaloes was carried out by ribotyping. The phenotypic characteriz.ation of the isolates revealed that all the isolates were homogenous in their biochemical profiles. The DIG labelled probe used for ribotypin8 was derived from the PCR amplification of 16S rRNA gene of P multocida strain P52. Ribotyping with Pst I revealed complete homogeneity among the isolates whereas EcoR rand Hind in was able to distinguish the isolates into 2 different ribotypes each.

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Author Biographies

  • A K ARORA
    Associate Professor,
  • S K JAND
    Profcssor cum Head,
    Department of Veterinary Microbiology,
  • K S SANDHU
    Professor cum Head,
    Department of Veterinary Epidemiology and Preventive Medicine;
  • M S OBEROI
    Dean, College of Veterinary Sciences,

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Issue

Vol. 74 No. 2 (2004)

Section

Articles

License

The copyright of the articles published in The Indian Journal of Animal Sciences is vested with the Indian Council of Agricultural Research, which reserves the right to enter into any agreement with any organization in India or abroad, for reprography, photocopying, storage and dissemination of information. The Council has no objection to using the material, provided the information is not being utilized for commercial purposes and wherever the information is being used, proper credit is given to ICAR.

How to Cite

ARORA, A. K., JAND, S. K., SANDHU, K. S., & OBEROI, M. S. (2014). Molecular characterization of Pasteurella multocida isolates from baemorrbagic septicaemia by ribotyping. The Indian Journal of Animal Sciences, 74(2). https://epubs.icar.org.in/index.php/IJAnS/article/view/38289