Genetic polymorphism of BoLA-DRB3.2 locus in crossbred cattle as revealed by PCR-RFLP analysis


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Authors

  • I GANGULY
  • A SARMA
  • S DE
  • A MITRA

Keywords:

BoLA-DRB3.2, Cattle, Gcnetic polymorphism, PCR-RHLP

Abstract

The present investigation was undertaken to explore DNA polymorphism in bovine class II DRB3.2 locus using polymerase chain reaction (peR) and restriction enzyme (RFLP) analysis. Genomic DNA was isolated from 21 Karan Fries (KF) and 20 Karan Swiss (KS) crossbred animals. A 304 bp fragment of the bovine lymphocyte antigen (BoLA)DRB3 gene-exon 2 was amplified by peR and the amplified product was digested independently with RsiYI, HaeIII and Rsa I enzymes. Digestion analysis revealed 3 BstYI, 6 HaeIII and 15 RsaI restriction patterns. All thesc patterns have previously been reported in cattle. In addition analysis of combined patterns of the 3 enzymcs resulted in 4 probable new alleles, viz. kba, tbb und tbe in KF and dba in KS.

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Author Biographies

  • I GANGULY
    Ph.D Scholar, 
    National Dairy Research Institute, Karnal, Haryana 132 001 India
  • A SARMA
    Head, National Dairy Research Institute, Karnal, Haryana 132 001 India

  • S DE
    Scientist, National Biotechnology Centre. National Dairy Research Institute, Karnal, Haryana 132 001 India

  • A MITRA
    Senior Scientist, Division of Animal Genetics, IVRl, Izatnagar 243 122.

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Issue

Vol. 74 No. 2 (2004)

Section

Articles

License

The copyright of the articles published in The Indian Journal of Animal Sciences is vested with the Indian Council of Agricultural Research, which reserves the right to enter into any agreement with any organization in India or abroad, for reprography, photocopying, storage and dissemination of information. The Council has no objection to using the material, provided the information is not being utilized for commercial purposes and wherever the information is being used, proper credit is given to ICAR.

How to Cite

GANGULY, I., SARMA, A., DE, S., & MITRA, A. (2014). Genetic polymorphism of BoLA-DRB3.2 locus in crossbred cattle as revealed by PCR-RFLP analysis. The Indian Journal of Animal Sciences, 74(2). https://epubs.icar.org.in/index.php/IJAnS/article/view/38343