Characterization of fowl adenoviruses isolated from field outbreaks of hydropericardium syndrome in Punjab


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Authors

  • AMARJIT SINGH
  • ANUPAMA KUMARI
  • N K MAITI
  • M S OBEROI

Keywords:

ELISA, Fowl adenovirus, Hydropericardium, Pathogenicity, Virus neutralization test, Virology

Abstract

Fowl adenovirus (FAV) isolates (LHPSV-2, 4, 6 and 7) obtained from field outbreaks of hydropcricardium syndrome (HPS) were characterized as serotype-4 of FAV using virus neutralization test. The pathogenicity of isolate LHPSY -7 was
studied in experimental birds. Clinical disease appeared on 3 days post inoculation (DPI) of HPS isolate (LHPSV-7) in 3-week-old disease free broiler chicks. In experimental birds 16.8% mortality was observed. The severity of disease was
seen between 5 and 7 DPI. The dead and sacrificed birds showed characteristic hydropericardium with transparent, amber coloured fluid in the pericardial cavity. Characteristic pathological changes in liver included, necrotic hepatitis and
intranuclear inclusion bodies in hepatocytes. The inclusions were also observed in kidneys, heart, spleen and pancreas at
variable stages of infection. Microscopically, depletion of lymphoid cells was observed in bursa, thymus, spleen and caecal tonsils. FAY antigen was detected at 7,13 and 21 DPI in plasma by antigen capture ELISA. Antibodies to FAV were
detected in serum by AGPT and indirect ELISA from 7 DPI onwards. A high titre (GMT> 1:64) was seen first on 3 DPI
followed by a dcerease in antibody titre, which again increased by 21 DPI (GMT> 1:53.81).

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Author Biographies

  • AMARJIT SINGH
    Associate Professor, Department of Veterinary Pathology
  • ANUPAMA KUMARI
    Research Assistant, Punjab Veterinary Vaccine Institute;
  • N K MAITI
    Senior Scientist, Microbiology, Central Institute of Freshwater Aquuculture. P. O. Kausalyaganga, Bhubaneshwar, Orissa 751 002
  • M S OBEROI
    Dean, College of veterinary Science. Punjab Agricultural University, Ludhiana, Punjab 141 004 India

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Issue

Vol. 74 No. 5 (2004)

Section

Articles

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How to Cite

SINGH, A., KUMARI, A., MAITI, N. K., & OBEROI, M. S. (2014). Characterization of fowl adenoviruses isolated from field outbreaks of hydropericardium syndrome in Punjab. The Indian Journal of Animal Sciences, 74(5). https://epubs.icar.org.in/index.php/IJAnS/article/view/38553